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med24

mediator complex subunit 24

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GeneInformationForm
GeneName med24
Aliases ENSDARG:ENSDARG00000032459; thrap4; fc05c04; trap100; wu:fc05c04; med24
Description mediator complex subunit 24
GenomicLocation chromosome 12 8822180-8854582 reverse strand
ExternalIDs Entrez:323621; UniGene:117152; ZFIN:ZDB-GENE-030131-2341;
TranscriptID ENSDART:ENSDART00000106375
mRNA NCBI:NM_001039062
GeneDescription Trap100 is one of the subunits of TRAP/mediator transcriptional regulation complex. A point mutation in trap100 causes a premature stop codon that truncates the protein causing a loss of function.
GeneFunction zebrafish trap100 is required for the proliferation of enteric precursors within the embryonic intestine.Pietsch et al., 2006 showed the requirement of trap100 for the normal development of cranial neural crest-derived structures. During development, trap100 is expressed in a dynamic tissue-specific expression pattern consistent with its function in ENS and jaw cartilage development.
GeneCloning

GeneStructure Med24 encodes a single transcript ENSDART00000050330 which consists of 13 exons and 1,707 bps. The protein product ENSDARP00000050329 consists of 568 residues.
Protein ENSDARP00000097153
ProteinDomainandFamilies has domain
Motifs has motif UniProt:Q2PW47
Expression ArrayExpress:ENSDARG00000032459;
GeneOntology

Orthologs

VariationAndRepeats

DisordersAndMutations Du¨rr et al. (2006) demonstrated m885 zebrafish mutation that disrupts crsp34/med27 subunit of mediator complex. They explored the role of mediator in controlling retinal differentiation by employing two additional mutations disrupting the mediator subunits Trap100/Med24 and crsp150/Med14. Their analysis showed, decrease in amacrine cell number with the loss of crsp34/Med27 but it increases the number of rod photoreceptor cells. In contrast, loss of Trap100/Med24 decreases rod photoreceptor cells. Their data thus provide evidence for differential requirements for Crsp34/Med27 in developmental processes.

Pietsch et al. (2006) predicted the lsn mutation in a trap100-null phenotype as lsnTrap100 protein that lacks all its predicted functional domains. Two different morpholino injections, a translation blocking morpholino or a splice-blocking morpholino, into lsn mutants did not resulted in a more severe phenotype that indicates lsn as a complete loss-of-function mutant. Thus, Trap100 is required for the normal proliferation of ENS precursors, but not for the initial specification and migration of the ENS precursors to the anterior end of the intestine.
RelatedPubMedArticles Pietsch, J.; Delalande, J. M.; Jakaitis, B.; Stensby, J. D.; Dohle, S.; Talbot, W. S.; Raible, D. W.; Shepherd, I. T.: lessen encodes a zebrafish trap100 required for enteric nervous system development. Development. 2006 Feb;133(3):395-406. Epub 2006 Jan 5. PMID:16396911

Dürr, K.; Holzschuh, J.; Filippi, A.; Ettl, A. K.; Ryu, S.; Shepherd, I. T.; Driever, W.: Differential roles of transcriptional mediator complex subunits Crsp34/Med27, Crsp150/Med14 and Trap100/Med24 during zebrafish retinal development. Genetics. 2006 Oct;174(2):693-705. Epub 2006 Apr 2. PMID:16582438 NCBI Resource Coordinators.: Database resources of the National Center for Biotechnology Information. Nucleic Acids Res. 41(Database issue):D8-D20. 2013. PMID:23193264
Kersey, P. J.; Allen, J. E.; Christensen, M.; et al.: Ensembl Genomes 2013: scaling up access to genome-wide data. Nucleic Acids Res. 2013. PMID:24163254
Sigrist, C. J. A.; de, Castro, E; Cerutti, L; Cuche, B. A.; Hulo, N.; Bridge, A.; Bougueleret, L. Xenarios, I.: New and continuing developments at PROSITE. Nucleic Acids Res. doi: 10.1093/nar/gks1067. 2012. PMID:23161676
Punta, M.; Coggill, P. C.; Eberhardt, et al.: The Pfam protein families database. Nucleic Acids Res. 40(Database Issue):D290-D301. 2012. PMID:22127870
Hunter, S.; Jones P.; Mitchell A.; et al.: Interpro in 2011: new developments in the family and domain prediction database. Nucleic Acids Res. doi: 10.1093/nar/gkr948. 2011. PMID:22096229
Carbon, S.; Ireland, A.; Mungall, C. J.; Shu, S.; Marshall, B.; Lewis, S.; AMIGO Hub; Web Presence Working Group.: AMIGO: online access to ontology and annotation data. Bioinformatics. 25(2):288-9. 2009. PMID:19033274
Ashburner, M.; Ball, C. A.; Blake, J. A.; et al. The Gene Ontology Consortium.: Gene ontology: tool for the unification of biology. Nat. Genet. 25(1):25-9. 2000. PMID:10802651
Sherry, S. T.; Ward, M. H.; Kholodov, M.; Baker, J.; Phan, L.; Smigielski, E. M.; Sirotkin, K.: dbSNP: the NCBI database of genetic variation. Nucleic Acids Res. 1;29(1):308-11. 2001. PMID:11125122
Bradford, Y.; Conlin, T.; Dunn, N.; et al.: ZFIN: enhancements and updates to the zebrafish model organism database. Nucleic Acids Res. 39(suppl 1):D822-D829. 2011. PMID:21036866
Kapushesky, M.; Adamusiak, T.; Burdett, T.; et al.: Gene Expression Atlas update--a value-added database of microarray and sequencing-based functional genomics experiments. Nucleic Acids Res. 40(Database isue):D1077-81. 2012. PMID:22064864

Web resources:
NCBI: http://www.ncbi.nlm.nih.gov/
PFAM: http://pfam.sanger.ac.uk/
PROSITE: http://prosite.expasy.org/
Interpro: http://www.ebi.ac.uk/interpro/
ZFIN: http://zfin.org/
Expression Atlas (EMBL): http://www.ebi.ac.uk/gxa/
Ensembl: http://asia.ensembl.org/Danio_rerio/
Database of Single Nucleotide Polymorphisms (dbSNP). Bethesda (MD): National Center for Biotechnology Information, National Library of Medicine.: http://www.ncbi.nlm.nih.gov/SNP/
PRINTS from Genomenet: http://www.genome.jp/
European Nucleotide Archive: http://www.ebi.ac.uk/ena/home
UNIGENE: http://www.ncbi.nlm.nih.gov/unigene/
AMIGO Gene Ontology: http://amigo.geneontology.org
Topic revision: r4 - 2009-06-14 - JayantMaini
 
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