|GeneName||IKAROS family zinc finger 1|
|Aliases||ENSDARG:ENSDARG00000013539; ikaros, znfn1a1|
|Description||IKAROS family zinc finger 1 (Ikaros)|
|GenomicLocation||chromosome 13 15911857-15948447 reverse strand|
|ExternalIDs||Entrez:30177; EMBL:AF092175; UniGene:8122; ZFIN:ZDB-GENE-980526-304;|
|TranscriptID||ENSDART:ENSDART00000016430; ENSDART:ENSDART00000042377; ENSDART:ENSDART00000046079; ENSDART:ENSDART00000050481; ENSDART:ENSDART00000079724|
Ikzf1 gene binds and activates the enhancer (delta-A element) of the CD3-delta gene. Functions in|
the specification and the maturation of the T-lymphocyte. Also interacts with a critical control
element in the TDT (terminal deoxynucleotidyltransferase) promoter as well as with the promoters for other genes expressed during early stages of B- and T-cell development
Willett et al. (2001) found that Ikaros was found to be most highly expressed in the thymus and pronephros , which is consistent with the differentiation of T and B cells, respectively, in these organs (Willett et al., 1997b, 1999; Danilova et al.,(2000). During development in zebrafish, Ikaros expression begins in lateral mesoderm, and continues in the intermediate cell mass (ICM), which is derived from lateral mesoderm and has been shown to generate primitive hematopoietic precursors. Ikaros-expressing cells were also found in the pharyngeal region, near the forming thymus. Later, such cells were seen in the pronephros, the site of hematopoiesis in adult fish. The timing of appearance of Ikaros-expressing cells suggests that, similar to other vertebrates, lymphocytes in the thymus arise from hematopoietic tissue located near the dorsal aorta or in the ICM.|
Lam et al. (2003) in this study, they have extended investigation on the maturation of the zebrafish immune system. The expression of immune-related genes of the zebrafish was monitored like ikaros, which encodes a transcription factor which is used as an early lymphoid marker, rag-1 etc to correlate the immune-related gene expression profiles,the in situ detection of cells expressing these genes in lymphoid tissues, and the detection of Ig and the on set of humoral immunity, and enable a better understanding of the different maturation state of theimmune system during zebrafish development.They found that Ikaros expression was first detected at 1 day post-fertilization (dpf) and thereafter increased gradually to more than two-fold between 28 and 42 dpf before decreasing to less than the initial 1 dpf expression level in adult fish (aged 105 dpf).
Murayama et al. (2006) by using an in situ photoactivatable cell tracer in the transparent zebrafish embryo, they demonstrated that definitive blood precursors appeared between the dorsal aorta and axial vein, validating the homology of this tissue with the AGM (aorta-gonadmesonephros) of amniotes. These cells first migrated through the blood to a previously undescribed caudal hematopoietic tissue (CHT), where they differentiated, expanded, and further migrated to seed the definitive hematopoietic organs, the thymus and kidney. Immigrants on the way to the thymus expressed c-myb and ikaros but not rag1; they were probably no longer HSCs, however, because they lacked scl and runx1 expression, unlike immigrants to the kidney.
Schorpp et al. (2006) examined the role of the transcription factor Ikaros in zebrafish lymphopoiesis. The phenotype of zebrafish homozygous for a recessive ikaros allele containing a nonsense mutation in the last coding exon was found to be similar to the situation of a loss-of-function mutation in the mouse. In fish larvae homozygous for an ikaros allele predicted to lack the C-terminal zinc fingers, T lymphopoiesis is absent; the presence of VHD_J_ rearrangements in adolescent fish is delayed in mutants. In adolescent mutant fish, T cells expressing tcrb and tcrd and B cells expressing igm are formed with low efficiency and display an oligoclonal Ag receptor repertoire.
|GeneCloning||This gene is contained in BAC clone CH211-57F7 (Vector:pTARBAC2.1)|
This gene encodes 5 transcripts. |
(ENSDART00000079724) is a novel transcript. It consists of 8 exons and is 1,915 bps in length. The protein product (ENSDARP00000074176) consists of 526 residues.
(ENSDART00000016430) consists of 9 exons and is 1,948 bps in length. The protein product (ENSDARP00000013960) consists of 537 residues.
(ENSDART00000042377) consists of 4 exons and is 1,219 bps in length. The protein product (ENSDARP00000042376) consists of 294 residues.
(ENSDART00000046079) consists of 6 exons and is 1,513 bps in length. The protein product (ENSDARP00000046078) consists of 392 residues.
(ENSDART00000050481) consists of 7 exons and is 1,639 bps in length. The protein product (ENSDARP00000050480) consists of 434 residues.
|Protein||ENSDARP00000013960 ENSDARP00000042376 ENSDARP00000046078 ENSDARP00000050480 ENSDARP00000074176|
|ProteinDomainandFamilies||has domain InterPro:IPR013087; InterPro:IPR001412; InterPro:IPR015880; InterPro:IPR007087;|
|Motifs||has motif Prosite:PS00028; Prosite:PS00178; PFAM:PF00096; PRINTS:PR00048;|
|GeneOntology||GO:0005622; GO:0006418; GO:0006829; GO:0003676; GO:0005524; GO:0008270; GO:0004812; GO:0046872;|
|VariationAndRepeats||RSID:rs40885506; RSID:rs40885506; RSID:rs40885506; RSID:rs40885506; RSID:rs40885506; RSID:rs41244902; RSID:rs41244902; RSID:rs41244902; RSID:rs41244902; RSID:rs41244902; RSID:rs41130272; RSID:rs41130272; RSID:rs41130272; RSID:rs41130272; RSID:rs41130272; RSID:rs41130907; RSID:rs41130907; RSID:rs41130907; RSID:rs41130907; RSID:rs41130907; RSID:rs40757200; RSID:rs40757200; RSID:rs40757200; RSID:rs40757200; RSID:rs40757200; RSID:rs41243819; RSID:rs41243819; RSID:rs41243819; RSID:rs41243819; RSID:rs41243819; RSID:rs40687973; RSID:rs40687973; RSID:rs40687973; RSID:rs40687973; RSID:rs40687973; RSID:rs40968736; RSID:rs40968736; RSID:rs40968736; RSID:rs40968736; RSID:rs40968736; RSID:rs40978066; RSID:rs40978066; RSID:rs40978066; RSID:rs40978066; RSID:rs40978066|
|DisordersAndMutations||ZFINID:ZDB-GENO-070827-1; ZFINID:ZDB-GENO-061212-1; Ikaros is an ENU based mutant, affected allel is it24980.|
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Expression Atlas (EMBL): http://www.ebi.ac.uk/gxa/
Database of Single Nucleotide Polymorphisms (dbSNP). Bethesda (MD): National Center for Biotechnology Information, National Library of Medicine.: http://www.ncbi.nlm.nih.gov/SNP/
PRINTS from Genomenet: http://www.genome.jp/
European Nucleotide Archive: http://www.ebi.ac.uk/ena/home
AMIGO Gene Ontology: http://amigo.geneontology.org